Clonal variation in Eucalyptus with Reference to Biochemical Parameters

Ranjani Vijayakumar, Chelliah Buvaneswaran, Rekha R Warrier, Rathinam Saviour Caesar Jayaraj
1.252 464

Abstract


The Institute of Forest Genetics and Tree Breeding, Coimbatore is involved in Eucalyptus tree improvement programme and identified highly productive on the basis of biometrical parameters. In the present study, 25 of these selected clones were evaluated for certain biochemical parameters €“ CA activity, chlorophyll and organic acids which may aid in future breeding programme as well as may act as markers. Results revealed that there exists highly significant variation in CA activity among the selected Eucalyptus clones. Among clones, clone - c196 registered the least and clone - c198 registered the greater values of chlorophyll-a, chlorophyll-b and total chlorophyll. ANOVA on organic acids revealed that there exist highly significant variations among the clones of eucalyptus. Among the organic acids studied, Oxalic acid registered the greater variation (CV of 10.63%) and Fumaric acid registered the least variation (CV of 5.60). In the present study, clustering analysis was carried out for grouping the related clones with reference to biochemical traits. The results revealed that these 25 selected clones formed three major groups. Among the biochemical parameters used for clustering of clones, Fumaric acid, followed by Oxalic acid and total chlorophyll mainly determined the clustering of selected eucalyptus clones.


Keywords


Eucalyptus, Carbonic anhydrase, Fumaric acid, Oxalic acid, Coimbatore

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References


Mendel, Z., A. Protasov, N. Fisher and J. La Salle. 2004. Taxonomy and biology of Leptocybe invasa gen. sp. n. (Hymenoptera: Eulophidae), an invasive gall inducer on Eucalyptus, Australian Journal of Entomology, 43:101-113.

Wilbur, K. and Anderson, N. (1948). Electrical and colorimetric determination of CA. J. Biochem. 176: 147.




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